|
KMID : 0379119880160040259
|
|
Korean Journal of Mycology
1988 Volume.16 No. 4 p.259 ~ p.259
|
|
|
Formation and Regeneration of protoplasts from phytophthora capsici mycelium
|
|
Lee Min-Woong
Nam Chung-Gu
|
|
|
Abstract
|
|
|
To find an appropriate enzyme system, 3 commercially available enzyme preparations(Cellulase from Tricoderma, sp. Macerozyme from Rhizopus sp. and Driselase from Basidiomycetes.) wete tested for lytic activity against P. capsici mycelium. some lytic activity was observed after Zhours incubation with cellulase and driselase. But macerozyme did not yield liberation of protoplast from P. capsici.
The enzyme mixtures containing cellulase $quot;Onozuka$quot; R-10(20 ㎎/㎖) macerozyme R-10(5 ㎎/㎖), driselase(10㎎/㎖) were yielded 1.2×10^7 protoplasts per 1g of P. capsici mycelium. Mannitol, osmotic stabilizer, was considerably effectivy to release protoplasts from P. capsici. The maximum release of protoplasts was attained after treatment of enzyme for 2hours at mycelium incubated for 2days. The number of nucleus of protoplast was usually from 3 to 7. The best medium to regenerate protoplasts was P.D.A. medium. When protoplasts diluted adequately were plated on the P.D.A. medium they formed bud of germ tube about 36%. The best osmotic stabilizer and pH to regenerate P. capsici protoplasts were 0.6 M mannitol and pH 6.0. P. capsici protoplasts were not regenerated on the media with kcl.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|